qScript cDNA Synthesis Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	When required, cDNA product can be diluted with 10 mM Tris-HCl (pH 8.0), 0.1 mM EDTA

Protocol tips
When required, cDNA product can be diluted with 10 mM Tris-HCl (pH 8.0), 0.1 mM EDTA

Publication protocol

cDNA synthesis was performed using 4 different commercial kits: Super Script® VILO™ (Invitrogen), RevertAaid™ First Strand cDNA Synthesis kit (Fermentas), iScript™ cDNA synthesis (Bio-Rad) and qScript™ cDNA Synthesis (Quanta BioSciences, Gaithersburg, MD, US) following the manufacturer's instructions. The same amount of total RNA (500 ng/20 µL) was reverse transcribed in two reaction volumes: 20 µL, as described by the manufacturer, and 10 µL. To determine the possibility of genomic DNA carry-over, control reactions were performed under the same conditions but lacking the reverse transcriptase enzyme (no-RT control). All RNA extracted was absent of significant genomic DNA, as determined by an average cycle threshold difference of 18.5±3.5 , equivalent to a maximum quantification error of 0.0003%.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing cDNA synthesis Bacteria using qScript cDNA Synthesis Kit from Quantabio.

Manufacturer protocol

Download the product protocol from Quantabio for qScript cDNA Synthesis Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing cDNA synthesis Bacteria using qScript cDNA Synthesis Kit from Quantabio. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.