RevertAid First Strand cDNA Synthesis Kit

Protocol tips

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	Add reaction mix and incubate at 37°C for 30 min. Add 1 µL 50 mM EDTA and incubate at 65°C for 10 min.

Protocol tips
Add reaction mix and incubate at 37°C for 30 min. Add 1 µL 50 mM EDTA and incubate at 65°C for 10 min.

Publication protocol

cDNA synthesis was performed using 4 different commercial kits: Super Script® VILO™ (Invitrogen), RevertAaid™ First Strand cDNA Synthesis kit (Fermentas), iScript™ cDNA synthesis (Bio-Rad) and qScript™ cDNA Synthesis (Quanta BioSciences, Gaithersburg, MD, US) following the manufacturer's instructions. The same amount of total RNA (500 ng/20 µL) was reverse transcribed in two reaction volumes: 20 µL, as described by the manufacturer, and 10 µL. To determine the possibility of genomic DNA carry-over, control reactions were performed under the same conditions but lacking the reverse transcriptase enzyme (no-RT control). All RNA extracted was absent of significant genomic DNA, as determined by an average cycle threshold difference of 18.5±3.5 , equivalent to a maximum quantification error of 0.0003%.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RevertAid First Strand cDNA Synthesis Kit below.

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