RevertAid First Strand cDNA Synthesis Kit

cDNA synthesis Bacteria

Experiment
cDNA synthesis Bacteria
Product
RevertAid First Strand cDNA Synthesis Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Add reaction mix and incubate at 37°C for 30 min.

Add 1 µL 50 mM EDTA and incubate at 65°C for
10 min.

Publication protocol

cDNA synthesis was performed using 4 different commercial kits: Super Script® VILO™ (Invitrogen), RevertAaid™ First Strand cDNA Synthesis kit (Fermentas), iScript™ cDNA synthesis (Bio-Rad) and qScript™ cDNA Synthesis (Quanta BioSciences, Gaithersburg, MD, US) following the manufacturer's instructions. The same amount of total RNA (500 ng/20 µL) was reverse transcribed in two reaction volumes: 20 µL, as described by the manufacturer, and 10 µL. To determine the possibility of genomic DNA carry-over, control reactions were performed under the same conditions but lacking the reverse transcriptase enzyme (no-RT control). All RNA extracted was absent of significant genomic DNA, as determined by an average cycle threshold difference of 18.5±3.5 , equivalent to a maximum quantification error of 0.0003%.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing cDNA synthesis Bacteria using RevertAid First Strand cDNA Synthesis Kit from Thermo Fisher Scientific.

Paper title
Optimizing a qPCR Gene Expression Quantification Assay for Biofilms: A Comparison between Commercial Kits and a Customized Protocol
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RevertAid First Strand cDNA Synthesis Kit below.

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