GoScript™ Reverse Transcriptase

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Reliable for Sensitive transcription of both high-copy and low-copy messages. Reverse Transcriptase is active across a range of 37–55°C, with greatest activity at 37–42°C

Upstream tips
Reliable for Sensitive transcription of both high-copy and low-copy messages. Reverse Transcriptase is active across a range of 37–55°C, with greatest activity at 37–42°C

Publication protocol

RNA extracts were treated with RiboLock RNase inhibitor (final concentration, 1 U μl−1; Fermentas) prior to reverse transcription-PCR. For synthesis of cDNA from extracted RNA, the GoScript reverse transcription system was employed according to the protocol of the manufacturer (Promega, Madison, WI) using random hexamers.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing cDNA synthesis Bacteria using GoScript™ Reverse Transcriptase from Promega.

Manufacturer protocol

Download the product protocol from Promega for GoScript™ Reverse Transcriptase below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing cDNA synthesis Bacteria using GoScript™ Reverse Transcriptase from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.