PrimeScript™ 1st strand cDNA Synthesis Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	After DNAse treatment add Oligo dT Primer,dNTP Mixture,Template RNA and RNase-free dH O and heat at 65°C for 5 min. Add reaction mix to the above mix and incubate at 30°C 10 min* and 42C for 60 min

Protocol tips
After DNAse treatment add Oligo dT Primer,dNTP Mixture,Template RNA and RNase-free dH O and heat at 65°C for 5 min. Add reaction mix to the above mix and incubate at 30°C 10 min* and 42C for 60 min

Publication protocol

First-strand cDNA was reverse-transcribed for each muscle sample using the PrimeScript First Strand cDNA Synthesis Kit (Takara, Tokyo, Japan) according to the manufacturer’s protocol. One microgram of total RNA, random primers, and a dNTP mixture in a 10-μL total reaction volume were incubated at 65 °C for 5 min, followed by quick-cooling on ice. Then, 5× PrimeScript buffer, RNase inhibitor, and PrimeScript RTase were added to a 20-μL total reaction volume, which was incubated at 30 °C for 10 min, followed by 42 °C for 60 min. Finally, the reverse transcript reaction mixture was heated to 95 °C for 5 min to stop the reaction.

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Manufacturer protocol

Download the product protocol from Takara Bio Inc for PrimeScript™ 1st strand cDNA Synthesis Kit below.

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