In situ apoptosis detection

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	To assess any potential toxicity of the miR-542-3p mimic transfection, apoptosis and necrosis were quantified by direct determination of nucleosomal DNA fragmentation using the Apoptotic/Necrotic/Healthy Cells Detection Kit (Takara bio Inc., Japan) as per the manufacture’s instructions.

Protocol tips
To assess any potential toxicity of the miR-542-3p mimic transfection, apoptosis and necrosis were quantified by direct determination of nucleosomal DNA fragmentation using the Apoptotic/Necrotic/Healthy Cells Detection Kit (Takara bio Inc., Japan) as per the manufacture’s instructions.

Publication protocol

Cell apoptotic/Necrotic detection assay:

To assess any potential toxicity of the miR-542-3p mimic
transfection, apoptosis and necrosis were quantified by direct determination of nucleosomal DNA fragmenta-
tion using the Apoptotic/Necrotic/Healthy Cells Detection Kit (Takara bio Inc., Japan) as per the manufacture’s instructions.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Apoptosis assay cell type - Human endometrial stromal cells using In situ apoptosis detection from Takara Bio Inc.

Manufacturer protocol

Download the product protocol from Takara Bio Inc for In situ apoptosis detection below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Apoptosis assay cell type - Human endometrial stromal cells using In situ apoptosis detection from Takara Bio Inc. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.