Tetro cDNA Synthesis Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
This kit is optimized for RT reactions using a wide range of total RNA amounts (10 pg -2 μg), such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis.	Incubate samples at 45 °C for 30 min. Incubate samples at 45 °C for 30 min	The use of higher incubation temperatures up to 48 °C may increase the yield of cDNA synthesized in cases of complex RNA secondary structure

Upstream tips
This kit is optimized for RT reactions using a wide range of total RNA amounts (10 pg -2 μg), such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis.
Protocol tips
Incubate samples at 45 °C for 30 min. Incubate samples at 45 °C for 30 min
Downstream tips
The use of higher incubation temperatures up to 48 °C may increase the yield of cDNA synthesized in cases of complex RNA secondary structure

Publication protocol

Total RNA was isolated from cecal tissue using RNeasy Mini Kit (Qiagen, Hilden, Germany) and cDNA was generated using the tetro cDNA synthesis kit (Bioline USA Inc. USA). Mouse angiogenin-4 and IL-13 gene expression was measured by real-time PCR using Sybr green with normalization to expression of the mouse house keeping genes βActin and GAPDH. IL-13 primers were purchased from Qiagen (Hilden, Germany). Angiogenin-4 primer sequences were: Angiogenin-4 forward: 5’- TTGGCTTGGCATCATAGT -3’, Angiogenin-4 reverse: 5’- CCAGCTTTGGAATCACTG -3’, Data were normalized with house keeping gene βActin; βActin Forward: 5’- AGCCATGTACGTAGCCATCC-3’, βActin Reverse: 5’-CTCTCAGCTGTGGTGGTGAA -3’, and GAPDH; GAPDH Forward: 5’-TGCACCACCAACTGCTTAGC -3’, GAPDH Reverse: 5’-GGCATGGACTGTGGTCATGAG -3’. Primers were purchased from Integrated DNA Technologies Coralville, Iowa, USA.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing cDNA synthesis Tissue using Tetro cDNA Synthesis Kit from Bioline.

Manufacturer protocol

Download the product protocol from Bioline for Tetro cDNA Synthesis Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing cDNA synthesis Tissue using Tetro cDNA Synthesis Kit from Bioline. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.