miRCURY RNA Isolation Kit

RNA isolation / purification Cells - immortalized HeLa

Experiment
RNA isolation / purification Cells - immortalized HeLa
Product
miRCURY RNA Isolation Kit from Exiqon
Manufacturer
Exiqon

Protocol tips

Protocol tips
the purified mitochondria were resuspended in RNA lysis buffer and incubated at room temperature in column for 5 min to allow binding. The column was washed thrice with washing buffer at 14000 rpm for 1 min. The RNA was eluted in nuclease free water from the column. Purity and concentration of RNA was determined using NanoPhotometer (Implen, Germany). The integrity of mitochondrial and total cellular RNA was checked with Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA).

Publication protocol

the purified mitochondria were resuspended in RNA lysis buffer and incubated at room temperature in column for 5 min to allow binding. The column was washed thrice with washing buffer at 14000 rpm for 1 min. The RNA was eluted in nuclease free water from the column. Purity and concentration of RNA was determined using NanoPhotometer (Implen, Germany). The integrity of mitochondrial and total cellular RNA was checked with Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA).

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized HeLa using miRCURY RNA Isolation Kit from Exiqon.

Paper title
Systematic Analysis of Small RNAs Associated with Human Mitochondria by Deep Sequencing: Detailed Analysis of Mitochondrial Associated miRNA
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Manufacturer protocol

Download the product protocol from Exiqon for miRCURY RNA Isolation Kit below.

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Videos

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