NucleoSpin® RNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	After pre-incubation with or without MAPK inhibitors followed by stimulation with or without 10 µM HA (as indicated) for 4 h, the medium was removed and the cells were washed twice with PBS. Total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's protocol.

Protocol tips
After pre-incubation with or without MAPK inhibitors followed by stimulation with or without 10 µM HA (as indicated) for 4 h, the medium was removed and the cells were washed twice with PBS. Total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's protocol.

Publication protocol

After pre-incubation with or without MAPK inhibitors followed by stimulation with or without 10 µM HA (as indicated) for 4 h, the medium was removed and the cells were washed twice with PBS. Total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's protocol.

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Manufacturer protocol

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