NucleoSpin® RNA

RNA isolation / purification Cells - immortalized HEK 293

Experiment
RNA isolation / purification Cells - immortalized HEK 293
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Protocol tips
After pre-incubation with or without MAPK inhibitors followed by stimulation with or without 10 µM HA (as indicated) for 4 h, the medium was removed and the cells were washed twice with PBS. Total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's protocol.

Publication protocol

After pre-incubation with or without MAPK inhibitors followed by stimulation with or without 10 µM HA (as indicated) for 4 h, the medium was removed and the cells were washed twice with PBS. Total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's protocol.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized HEK 293 using NucleoSpin® RNA from Macherey Nagel.

Paper title
Distinct Signalling Pathways of Murine Histamine H- and H-Receptors Expressed at Comparable Levels in HEK293 Cells
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Manufacturer protocol

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