RediPlate™ 96 RiboGreen™ RNA Quantitation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Quantitation Range is 3-200 ng.	Add 180 µL of the provided RediPlate TE buffer (Component B) to samples. Incubate samples for 10 minutes at room temperature, protected from light.

Upstream tips
Quantitation Range is 3-200 ng.
Protocol tips
Add 180 µL of the provided RediPlate TE buffer (Component B) to samples. Incubate samples for 10 minutes at room temperature, protected from light.

Publication protocol

An amount of 100 ng RNA from each sample (PFC n = 47 and CER n = 48) was biotinylated and amplified using the Illumina TotalPrep RNA Amplification kit (Life Technologies) to produce cRNA. cRNA was quantitated using a NanoDrop NO-1000 (Thermo Fisher Scientific) and RediPlate 96 RiboGreen RNA Quantitation Kit (Life Technologies). Genome-wide expression was assessed using the Illumina HumanHT-12 v4 Expression BeadChip (Illumina Inc.) according to manufacturer’s instruction.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RediPlate™ 96 RiboGreen™ RNA Quantitation Kit below.

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