RediPlate™ 96 RiboGreen™ RNA Quantitation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Quantitation Range is 3-200 ng	Add 180 µL of the provided RediPlate TE buffer (Component B) to samples. Incubate samples for 10 minutes at room temperature, protected from light.

Upstream tips
Quantitation Range is 3-200 ng
Protocol tips
Add 180 µL of the provided RediPlate TE buffer (Component B) to samples. Incubate samples for 10 minutes at room temperature, protected from light.

Publication protocol

Total RNA was isolated from 50 to 100 mg of BAT and scWAT. Tissues were homogenized with QIAzol Lysis Reagent and TissueLyser LT according to the manufacturer’s instructions (Qiagen, Hilden, Germany). From BAT, miRNA-included total RNA was isolated with the miRNeasy Mini Kit, whereas from scWAT, total RNA and miRNA-enriched fraction were isolated separately with the RNeasy Mini Kit and the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany). The concentration and quality of the total RNA were analyzed with a NanoDrop ND-1000 UV-Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and the concentration of miRNA-enriched fraction with the RediPlate 96 RiboGreen RNA Quantitation Kit (Molecular Probes, Eugene, OR, USA). Genomic DNA was removed from the total RNA with DNAase treatment and cDNA was synthetized from 500 ng of total RNA using the RevertAid™ First Strand cDNA Synthesis Kit (Fermentas, Sankt Leon-Rot, Germany). From scWAT, miRNA cDNA synthesis was conducted from 100 ng of miRNA-enriched fraction while from BAT, miRNA cDNA was synthesized from 500 ng of total RNA with the qScript microRNA cDNA Synthesis Kit (Quanta BioSciences, Gaithersburg, MD, USA).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for RediPlate™ 96 RiboGreen™ RNA Quantitation Kit below.

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