Agilent RNA 6000 Pico Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Sensitive to detect 50 pg/µL in water or 200 pg/µL in TE of Total RNA and 250 pg/µL in water or 500 pg/µL in TE of mRNA Assay

Upstream tips
Sensitive to detect 50 pg/µL in water or 200 pg/µL in TE of Total RNA and 250 pg/µL in water or 500 pg/µL in TE of mRNA Assay

Publication protocol

3.2.3.2. Agilent Bioanalyzer, Eukaryotic RNA Pico Chip
Take out the reagents 30 min prior to run the Chip and allow them to RT in the dark.

Follow the manufacture instructions for preparing the gel-dye-matrix properly, and running the chip (45 min in total).

Criteria of QCs of extracted RNA
-Quant-iT Ribogreen RNA assay: if total RNA amount < 5 ng is not permitted to proceed with the library construction (RNA yield is about 50-80 ng/mL saliva).

-RNA 6000 Pico Chip, Bioanalyzer: detection of intact ribosomal RNA peak will make the exclusion of the sample for further analysis, since will evidence of residual cell contamination (eukaryotic: 18S (1869 nt), 28S rRNA (5070 nt); prokaryotic: 16S (1542 nt), 23S rRNA (2906 nt)).

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Manufacturer protocol

Download the product protocol from Agilent Technologies for Agilent RNA 6000 Pico Kit below.

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