Qubit RNA HS Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
The Qubit® assays are designed to be performed at room temperature, as temperature fluctuations can influence the accuracy of the assay.	Dilute Qubit® RNA HS Reagent 1:200 in Qubit® RNA HS Buffer.

Upstream tips
The Qubit® assays are designed to be performed at room temperature, as temperature fluctuations can influence the accuracy of the assay.
Protocol tips
Dilute Qubit® RNA HS Reagent 1:200 in Qubit® RNA HS Buffer.

Publication protocol

The RIP-seq experiment was conducted using two biological replicates for each sample. For the total repertoire of SIRT7 RIP-RNA, we sequenced both cytosolic and nuclear RNAs that were associated with Flag-SIRT7 respectively. In order to deep sequence the non-rRNA repertoire, rRNA was subtracted from both pre-RIP input RNA and RNA co-immunoprecipitated with Flag-SIRT7 using the RiboZero Magnetic Gold Human/Mouse/Rat Kit (Illumina). All RNA samples were treated with DNase I in solution to completely remove genomic DNA. RNA samples were quantified with a Qubit 2.0 (RNA HS kit; Thermo) and were then analyzed on the BioAnalyzer High Sensitivity DNA chip (Agilent) to determine RNA integrity before library construction.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit RNA HS Assay Kit below.

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