Quant-iT™ RiboGreen™ RNA Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
This kit enables quantitation of as little as 1 ng/mL	Add 1.0 mL of the aqueous working solution of the Quant-iT™ Ribo Green® reagent and incubate for 2 to 5 minutes at room temperature, protected from light.

Upstream tips
This kit enables quantitation of as little as 1 ng/mL
Protocol tips
Add 1.0 mL of the aqueous working solution of the Quant-iT™ Ribo Green® reagent and incubate for 2 to 5 minutes at room temperature, protected from light.

Publication protocol

RiboGreen analysis
We used the Quant-iT™ RiboGreen® RNA Assay Kit (Life Technologies, catalogue no. R11490) to determine the RNA yields from each 1.0 mL of CSF (n = 2 duplicates/kit). We utilised the low-range (0–50 pg/µL) protocol adopted for 200 µL samples in a 96-well format (Costar). The 96-well plates assays were read on a plate reader at each site. OHSU used a Molecular Devices SpectraMax M2 Plate Reader with SoftMax Pro 6 Analysis Software; TGen used a BioTek Cytation 3 Imaging Reader BioTek with Gen5 Software v. 2.06 BioTek; and UCSD used a Tecan Infinite 200 Plate Reader with Tecan i-control software v. 1.10.4.0.

Small RNA Bioanalyzer analysis
We used the Agilent Small RNA Assay (Agilent Technologies 2100 Bioanalyzer with 2100 Expert Software) to determine the yield and purity of RNA. Duplicate (n = 2) RNA yields from 1.0 mL of CSF were averaged.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Quant-iT™ RiboGreen™ RNA Assay Kit below.

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