pX330-U6-Chimeric_BB-CBh-hSpCas9

CRISPR Human - Repression EZH2

Experiment
CRISPR Human - Repression EZH2
Product
pX330-U6-Chimeric_BB-CBh-hSpCas9 from Addgene
Manufacturer
Addgene

Protocol tips

Publication protocol

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Discussion

Discussion

4 years ago

Author: Milena Alexeyeva Russian Federation

DNA insert using CRISPR

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

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Papers

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Manufacturer protocol

Download the product protocol from Addgene for pX330-U6-Chimeric_BB-CBh-hSpCas9 below.

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Videos

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