FOXA2 Human Gene Knockout Kit
CRISPR Human - Repression FOXA2
Protocol tips
| Downstream tips |
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| it is recommended that you still keep growing or freeze some of the transfected cells without selection; just in case, you need to perform the selection again. |
Publication protocol
CRISPR/Cas9 system.The FOXA2 human gene knockout kit via CRISPR was ordered from OriGene (KN204066). Clones were selected using 2 μg/ml puromycin.
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Reviews
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Discussion
Discussion
5 years ago
Author:
Milena Alexeyeva
DNA insert using CRISPR
I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?
Papers
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Manufacturer protocol
Download the product protocol from OriGene for FOXA2 Human Gene Knockout Kit below.
Download manufacturer protocol
Videos
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