|If transfecting more than one plasmid (i.e., CRISPR/Cas9 KO Plasmid with HDR Plasmid), mix Plasmid DNA at equivalent ratios.|
|Do not add antibiotics to the Plasmid Transfection Medium|
dCK CRISPR/Cas9 KO Plasmid (h) from Santa Cruz Biotechnology has not yet been reviewed for this experiment
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3 years ago
Author: Milena Alexeyeva
I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?
Check out relevant papers found by Labettor's AI that are relevant for performing CRISPR Human - Repression DCK using dCK CRISPR/Cas9 KO Plasmid (h) from Santa Cruz Biotechnology.
Download the product protocol from Santa Cruz Biotechnology for dCK CRISPR/Cas9 KO Plasmid (h) below.Download manufacturer protocol
Check out videos that might be relevant for performing CRISPR Human - Repression DCK using dCK CRISPR/Cas9 KO Plasmid (h) from Santa Cruz Biotechnology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
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