dCK CRISPR/Cas9 KO Plasmid (h)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
If transfecting more than one plasmid (i.e., CRISPR/Cas9 KO Plasmid with HDR Plasmid), mix Plasmid DNA at equivalent ratios.	Do not add antibiotics to the Plasmid Transfection Medium

Upstream tips
If transfecting more than one plasmid (i.e., CRISPR/Cas9 KO Plasmid with HDR Plasmid), mix Plasmid DNA at equivalent ratios.
Protocol tips
Do not add antibiotics to the Plasmid Transfection Medium

Publication protocol

CRISPR/Cas-9
1.5 × 105 SUIT2 or PANC-1 cells were seeded in 6-well plates and allowed to adhere overnight before adding 300μl transfection medium (Santa-Cruz) containing 3μl CRISPR Transfection Reagent (Santa-Cruz) and 2μg of control (Santa Cruz - sc-418922) or DCK CRISPR plasmid (Santa-Cruz - sc-417715). 24 hours following transfection, GFP positive cells were isolated by flow cytometry and cultured in DMEM media containing 20% FCS. Following recovery, cells were maintained under normal culture conditions and protein and cell viability assays performed as described above.

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Papers

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for dCK CRISPR/Cas9 KO Plasmid (h) below.

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