lentiCRISPR v2
CRISPR Human - Repression BCL6
Protocol tips
| Protocol tips |
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| There is no need to perform a negative control golden-gate reaction (without insert) as it will always contain colonies so not a good indicator of cloning success. |
Publication protocol
There is no need to perform a negative control golden-gate reaction (withoutinsert) as it will always contain colonies so not a good indicator of cloning success.
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Discussion
Discussion
4 years ago
Author:
Milena Alexeyeva
DNA insert using CRISPR
I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?
Papers
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Manufacturer protocol
Download the product protocol from Addgene for lentiCRISPR v2 below.
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Videos
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