pMXs-IRES-Bsd Retroviral Expression Vector

CRISPR Human - Repression DDX3Y

Experiment
CRISPR Human - Repression DDX3Y
Product
pMXs-IRES-Bsd Retroviral Expression Vector from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Upstream tips
To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip (20uL or 200uL one), puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate.

Publication protocol

Vector construction
The GFP and FLAG-tagged C16orf80, C3orf17, and C9orf114 expression vectors were
constructed by cloning, via Gibson assembly, cDNA inserts generated by PCR from a KBM7
cDNA library into versions of the pMXs-IRES-Bsd vector containing a C-terminal GFP or
FLAG tag. For sgDDX3Y rescue experiments, GFP and DDX3X expression vectors were
constructed by cloning, via Gibson assembly, cDNA inserts generated by PCR from a GFPtagged pMXs-IRES-Bsd vector (for GFP) and a KBM7 cDNA library into the pMXs-IRES-Bsd
vector (for DDX3X). For live-cell imaging, the EGFP cassette in pEGFP-C1-Fibrillarin was
replaced with turboRFP.

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Reviews

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Discussion

Discussion

4 years ago

Author: Milena Alexeyeva Russian Federation

DNA insert using CRISPR

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing CRISPR Human - Repression DDX3Y using pMXs-IRES-Bsd Retroviral Expression Vector from Cell Biolabs.

Paper title
Identification and characterization of essential genes in the human genome
Full paper
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Manufacturer protocol

Download the product protocol from Cell Biolabs for pMXs-IRES-Bsd Retroviral Expression Vector below.

Download PDF Download manufacturer protocol

Videos

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