pMXs-IRES-Bsd Retroviral Expression Vector

CRISPR Human - Repression DDX3Y

Experiment

CRISPR Human - Repression DDX3Y

Product

pMXs-IRES-Bsd Retroviral Expression Vector from Cell Biolabs

Manufacturer

Cell Biolabs

Buy product Write review

Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip (20uL or 200uL one), puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate.

Upstream tips
To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip (20uL or 200uL one), puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate.

Publication protocol

Vector construction
The GFP and FLAG-tagged C16orf80, C3orf17, and C9orf114 expression vectors were
constructed by cloning, via Gibson assembly, cDNA inserts generated by PCR from a KBM7
cDNA library into versions of the pMXs-IRES-Bsd vector containing a C-terminal GFP or
FLAG tag. For sgDDX3Y rescue experiments, GFP and DDX3X expression vectors were
constructed by cloning, via Gibson assembly, cDNA inserts generated by PCR from a GFPtagged pMXs-IRES-Bsd vector (for GFP) and a KBM7 cDNA library into the pMXs-IRES-Bsd
vector (for DDX3X). For live-cell imaging, the EGFP cassette in pEGFP-C1-Fibrillarin was
replaced with turboRFP.

Full paper Login or join for free to view the full paper.

Reviews

pMXs-IRES-Bsd Retroviral Expression Vector from Cell Biolabs has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

4 years ago

Author: Milena Alexeyeva Russian Federation

DNA insert using CRISPR

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Comment View 2 comments

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing CRISPR Human - Repression DDX3Y using pMXs-IRES-Bsd Retroviral Expression Vector from Cell Biolabs.

Paper title

Identification and characterization of essential genes in the human genome

Full paper

Manufacturer protocol

Download the product protocol from Cell Biolabs for pMXs-IRES-Bsd Retroviral Expression Vector below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing CRISPR Human - Repression DDX3Y using pMXs-IRES-Bsd Retroviral Expression Vector from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment