SuperLight™ Dual Luciferase Reporter Gene Assay Kit

Reporter gene assay luciferase - HepG2 and Huh7 cells

Experiment
Reporter gene assay luciferase - HepG2 and Huh7 cells
Product
SuperLight™ Dual Luciferase Reporter Gene Assay Kit from Bio Trend
Manufacturer
Bio Trend

Protocol tips

Protocol tips
Luciferase activity was assayed using SuperLightTM Luciferase Reporter Gene Assay and measured using a Spectra Max M3 microplate reader (Molecular Devices, Inc., Sunnyvale, CA).

Publication protocol

miR-mock, miR-18a, miR-138, miR-199a-5p, or miR-93 was co-transfected with 3′UTR luciferase vectors of the vehicle control and HIF1A 3′UTR in HepG2 cells for 48 h. Luciferase activity was assayed using SuperLightTM Luciferase Reporter Gene Assay and measured using a Spectra Max M3 microplate reader (Molecular Devices, Inc., Sunnyvale, CA).

Full paper   Login or join for free to view the full paper.

Reviews

SuperLight™ Dual Luciferase Reporter Gene Assay Kit from Bio Trend has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: J.Han China

Which transfection reagent would work better on stem cells?

I am planning on running reporter assays on stem cells and I would like to know which transfection reagent would work better?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay luciferase - HepG2 and Huh7 cells using SuperLight™ Dual Luciferase Reporter Gene Assay Kit from Bio Trend.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Bio Trend for SuperLight™ Dual Luciferase Reporter Gene Assay Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Reporter gene assay luciferase - HepG2 and Huh7 cells using SuperLight™ Dual Luciferase Reporter Gene Assay Kit from Bio Trend. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms