SuperLight™ Dual Luciferase Reporter Gene Assay Kit

Reporter gene assay luciferase - HepG2 and Huh7 cells

Experiment
Reporter gene assay luciferase - HepG2 and Huh7 cells
Product
SuperLight™ Dual Luciferase Reporter Gene Assay Kit from Bio Trend
Manufacturer
Bio Trend

Protocol tips

Protocol tips
Luciferase activity was assayed using SuperLightTM Luciferase Reporter Gene Assay and measured using a Spectra Max M3 microplate reader (Molecular Devices, Inc., Sunnyvale, CA).

Publication protocol

miR-mock, miR-18a, miR-138, miR-199a-5p, or miR-93 was co-transfected with 3′UTR luciferase vectors of the vehicle control and HIF1A 3′UTR in HepG2 cells for 48 h. Luciferase activity was assayed using SuperLightTM Luciferase Reporter Gene Assay and measured using a Spectra Max M3 microplate reader (Molecular Devices, Inc., Sunnyvale, CA).

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Discussion

Discussion

4 years ago

Author: J.Han China

Which transfection reagent would work better on stem cells?

I am planning on running reporter assays on stem cells and I would like to know which transfection reagent would work better?

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Papers

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Manufacturer protocol

Download the product protocol from Bio Trend for SuperLight™ Dual Luciferase Reporter Gene Assay Kit below.

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