Control siRNA-A

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA concentration-37.5 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C

Protocol tips
siRNA concentration-37.5 nM Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio). Incubate for 5 minutes at room temperature and add to cells. Incubate cells for 1–3 days at 37°C

Publication protocol

Small interfering RNA (siRNA) against the YAP gene (sc-38637; Santa Cruz Biotechnology Inc, Dallas, TX, USA) was used for loss-of-function experiments. The control siRNA sc-37007 (Santa Cruz Biotechnology) was used as a negative control. Each siRNA (37.5 nM) was transfected into ovarian cells using Lipofectamine RNAiMAX (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. The knockdown of a target gene was verified by Western blotting.

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Manufacturer protocol

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