Lipofectamine® RNAiMAX Transfection Reagent
siRNA / RNAi /miRNA transfection Human Cells - OV-2008 Lipofectamine
Protocol tips
| Protocol tips |
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| siRNA concentration of 50 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells fro 1-3 days at 37C |
Publication protocol
RNA oligonucleotides were transfected using Lipofectamine RNAiMAX in a final concentration of 100 nM miR-206 or miR613, 200 nM anti-miR-206 or anti miR-613. RNA transfection efficiency was approximately 70–80% and the overexpression of miRNA persisted for at least 4 days.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Keith L. Morrison
siRNA/RNAi/miRNA transfection human
I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?
Papers
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Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for Lipofectamine® RNAiMAX Transfection Reagent below.
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