CD38 CRISPR Activation Plasmid (r)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
If transfecting more than one plasmid (i.e., CRISPR/Cas9 KO Plasmid with HDR Plasmid), mix Plasmid DNA at equivalent ratios.	Do not add antibiotics to the Plasmid Transfection Medium

Upstream tips
If transfecting more than one plasmid (i.e., CRISPR/Cas9 KO Plasmid with HDR Plasmid), mix Plasmid DNA at equivalent ratios.
Protocol tips
Do not add antibiotics to the Plasmid Transfection Medium

Publication protocol

CRISPR activation plasmid transfection
Control CRISPR activation plasmid (sc-437275), rat CD38 CRISPR activation plasmid (sc-437321-ACT) were obtained from Santa Cruz Biotechnology. Transfection was performed according to the transfection protocol for cell cultures from Santa Cruz Biotechnology. Briefly, Plasmid transfection reagent mixture of 1ml (Transfection reagent, sc-395739, Transfection medium, sc-108062) was co-incubated with astrocytes for 24 hours in a 5% CO2 incubator at 37°C, and then CD38 cyclase activity was assessed in order to confirm efficiency of transfection.

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Papers

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for CD38 CRISPR Activation Plasmid (r) below.

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