SurePrint G3 Human CGH Microarray Kit, 4x180K

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not use DEPC-treated water, only water provided in the kit or nuclease-free water

Protocol tips
Do not use DEPC-treated water, only water provided in the kit or nuclease-free water

Publication protocol

Array CGH Analysis
Genomic copy-number analysis was performed by using the Agilent Human Genome CGH Microarray 180 K kit (Agilent Technologies, Palo Alto, CA, USA) by following the manufacturer's recommendations, with a Genomic DNA (Female Promega, Mannheim, Germany) as reference. The analysis was performed by using Feature Extraction v10.7 and DNA Analytics v6.5 software (Agilent Technologies) applying the ADM2 algorithm with a threshold of 5, minimum absolute average log2 ratio in called intervals of 0.30, and a minimum of three probes. Putative chromosome copy-number changes were defined by intervals of three or more adjacent probes and were considered to be duplicated or deleted when results exceeded the ±0.30 range. All nucleotide positions were referred to the Human Reference Sequence (GRCh37) Assembly Feb. 2009 hg19 of UCSC.

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Manufacturer protocol

Download the product protocol from Agilent Technologies for SurePrint G3 Human CGH Microarray Kit, 4x180K below.

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