Human Genome CGH Microarray Kit 244A

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not filter the Stabilization and Drying Solution	use the Stabilization and Drying Solution in the fume hood only

Upstream tips
Do not filter the Stabilization and Drying Solution
Protocol tips
use the Stabilization and Drying Solution in the fume hood only

Publication protocol

Arrays for Comparative Genomic Hybridization:
aCGH was performed using a high-density array system with 244,000 oligonucleotides on a chip (Cat. No. G4411B, Agilent, Waldbronn, Germany), covering the complete genome in an average of approximately 5–13 kB. DNA was isolated using the QIAamp DNA Mini kit, (Qiagen Cat. No. 51304), following the supplier’s instructions for cultured cells. Labeling and hybridization were also done as described by the
supplier. As a reference, DNA of a female donor was taken (Cat. No. G1521, Promega, Mannheim, Germany). Hybridization was performed for 40 h at 65 °C in a hybridization oven (Agilent G2545A).

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Manufacturer protocol

Download the product protocol from Agilent Technologies for Human Genome CGH Microarray Kit 244A below.

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