GeneChip™ Human Genome U133A 2.0 Array

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Avoid touching the surface of the glass with your fingers. Skin oils and other substances, such as lotions or ink, can fluoresce. If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Upstream tips
Avoid touching the surface of the glass with your fingers. Skin oils and other substances, such as lotions or ink, can fluoresce. If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Publication protocol

Oligonucleotide cDNA microarray analysis
RNA purification and hybridization for the microarray analyses were performed as previously described (Jiang, et al 2009). In brief, total RNA from patient tumour specimens was isolated by Trizol reagents (Invitrogen, Carlsbad, CA) and followed by affinity column purification (Qiagen, Valencia, CA). cDNA was generated from 100 ng total RNA using a poly dT oligonucleotide (Affymetrix, Santa Clara, CA). Biotinylated labelling of cRNA was performed by in vitro transcription according to manufacturer’s protocol. Fragmented cRNA (15 µg) was hybridized to HG_U133 A2 Gene Chip® arrays (Affymetrix) using Fluidics Station 450 (Affymetrix).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for GeneChip™ Human Genome U133A 2.0 Array below.

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