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Each labeled miPSC DNA was hybridized together with the labeled donor DNA onto Agilent SurePrint G3 mouse 1 × 1 M microarray for 40 hours at 65°C. DNA processing, microarray handling and scanning were conducted following the Agilent oligonucleotide CGH protocol (version 6.0). |
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| Protocol tips |
| Each labeled miPSC DNA was hybridized together with the labeled donor DNA onto Agilent SurePrint G3 mouse 1 × 1 M microarray for 40 hours at 65°C. DNA processing, microarray handling and scanning were conducted following the Agilent oligonucleotide CGH protocol (version 6.0). |
Publication protocol
Genomic DNAs extracted from each miPSC line and the parental donor (MEF B2) were fragmented using AluI and RsaI enzyme digestion. DNA labeling was conducted using Agilent SureTag DNA Labeling Kit. Different fluorescence dyes were used for DNA labeling of miPSCs (Cy5-dUTP) and the donor parental cell line (Cy3-dUTP). Each labeled miPSC DNA was hybridized together with the labeled donor DNA onto Agilent SurePrint G3 mouse 1 × 1 M microarray for 40 hours at 65°C. DNA processing, microarray handling and scanning were conducted following the Agilent oligonucleotide CGH protocol (version 6.0).
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Genome-wide CNV analysis in mouse induced pluripotent stem cells reveals dosage effect of pluripotent factors on genome integrity
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