CYTAG® CGH Labeling kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		Label all samples at one time to avoid day-to-day variations. The labeled samples can be stored at -80°C upto 3 months and successive hybridizations

Downstream tips
Label all samples at one time to avoid day-to-day variations. The labeled samples can be stored at -80°C upto 3 months and successive hybridizations

Publication protocol

ample labelling and microarray processing. Three hundred nanograms of each DNA sample was labelled with Cy-5 fluorescent dye using the CGH Labelling Kit for Oligo Arrays (Enzo Life Sciences Farmingdale, New York) according to the manufacturer’s protocol. As a reference genotype for hybridization 300 ng of Human Reference DNA – Male or Female (Agilent Technologies, Santa Clara, California) was simultaneously labelled with Cy-3 dye. Hybridization of samples and processing of microarrays were performed with the standard Agilent Oligonucleotide Array-Based CGH protocol.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTAG® CGH Labeling kit below.

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