Human Genome CGH Microarray Kit 244A

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Avoid touching the surface of the glass with your finersǯ Skin oils and other substances, such as lotions or ink, can fluoresceǯ If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Protocol tips
Avoid touching the surface of the glass with your finersǯ Skin oils and other substances, such as lotions or ink, can fluoresceǯ If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Publication protocol

Array CGH analysis
Array CGH analysis was conducted using the Human Genome CGH 244K Oligo Microarray Kit (G4411B, Agilent Technologies, Santa Clara, CA, USA). Genomic DNA prepared from primary neuroblastoma tissues or cell lines was labeled with Cy3-dye using a QuickAmp labeling kit. Human placental DNA was labeled with Cy5-dye and used as a reference control. Labeling, hybridization and subsequent data processing by FeatureExtraction and CGH-Analytics software were performed according to the manufacturer's instructions. Relative copy number of the probes surrounding the and genomic locus (from to ) were compared in each primary tumor or cell line.

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Manufacturer protocol

Download the product protocol from Agilent Technologies for Human Genome CGH Microarray Kit 244A below.

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