Human Genome CGH Microarray Kit, 4x44K

Microarray Comperative genomic hybridization - Human HeLa

Experiment
Microarray Comperative genomic hybridization - Human HeLa
Product
Human Genome CGH Microarray Kit, 4x44K from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Upstream tips
Avoid touching the surface of
the glass with your fingers. Skin oils and other substances, such as
lotions or ink, can fluoresce. If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Publication protocol

Microarray comparative genomic hybridization analysis
Microarray comparative genomic hybridization using Human Genome CGH Microarray Kit (4x44K) (Agilent Technologies, Santa Clara, CA, USA) was used for identifying chromosomal CNV of 6 cervical cancer cell lines and 3 normal cervical samples as per the manufacturer’s protocol. Briefly, genomic DNA of cervical cancer cell lines was extracted using a DNeasy Blood & Tissue Kit (QIAGEN, Cat No. 69506). 1 μg genomic DNA of test sample and 1 μg human sex-matched control DNA as a reference sample (Promega G1521A; Lot no. 20929604) were digested using Alu I and Rsa I. This was followed by fluorescent labeling, clean-up of labeled genomic DNA, microarray hybridization and scanning. The data were extracted using the Agilent Feature Extraction (FE) v11.0 program. After calculating the background signal, non-uniform signal and the average raw signal on each probe, the resulting data files were generated and transferred to the bioinformatics software, Nexus Copy Number version 6.1 (BioDiscovery, Inc., El Segundo, CA, USA) for analysis [6].



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Papers

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Paper title
Integrated analysis of chromosome copy number variation and gene expression in cervical carcinoma
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Manufacturer protocol

Download the product protocol from Agilent Technologies for Human Genome CGH Microarray Kit, 4x44K below.

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