SurePrint G3 Human CGH Microarray Kit, 2x400K

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Avoid touching the surface of the glass with your fingers. Skin oils and other substances, such as lotions or ink, can fluoresce. If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Upstream tips
Avoid touching the surface of the glass with your fingers. Skin oils and other substances, such as lotions or ink, can fluoresce. If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Publication protocol

Two PARK4 blood samples were assessed for copy number variation using a SurePrint G3 Human CGH (comparative genomic hybridization) microarray 2×400 K design 021850 (Agilent Technologies). Agilent's labeling kit was used, and all procedures were carried out according to the manufacturer's instructions. Scanning was performed on an Agilent microarray scanner and raw data were processed by Feature Extraction 9.5. Deleted and amplified regions were determined on Agilent's Genomic Workbench Standard Edition 5.0.14. A minimum of four consecutive probes had to be affected to make a decision. The aberration detection threshold of the ADM-2 algorithm was set to 5.9.

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Manufacturer protocol

Download the product protocol from Agilent Technologies for SurePrint G3 Human CGH Microarray Kit, 2x400K below.

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