Quant-iT™ PicoGreen® dsDNA Assay Kit

DNA quantification Human - SH-SY5Y

Experiment

DNA quantification Human - SH-SY5Y

Product

Quant-iT™ PicoGreen® dsDNA Assay Kit from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	To minimize photo bleaching effects, keep the time for fluorescence measurement constant for all samples. During incubation at room temperature, protected from light.

Protocol tips
To minimize photo bleaching effects, keep the time for fluorescence measurement constant for all samples. During incubation at room temperature, protected from light.

Publication protocol

The genomic DNA was sequenced by Illumina using the Illumina Fast Track Services (FTS) methodology:

gDNA Quantitation.

Genomic DNA is quantified prior to library construction using PicoGreen (Quant-iT™ PicoGreen dsDNA Reagent, Invitrogen, Catalog #: P11496). Quants are read with Spectromax Gemini XPS (Molecular Devices).

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Reviews

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Discussion

5 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - SH-SY5Y using Quant-iT™ PicoGreen® dsDNA Assay Kit from Thermo Fisher Scientific.

Paper title

Systems genomics evaluation of the SH-SY5Y neuroblastoma cell line as a model for Parkinson’s disease

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Quant-iT™ PicoGreen® dsDNA Assay Kit below.

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Videos

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