Hoechst 33258

DNA quantification Human - MDA-MB-231

Experiment
DNA quantification Human - MDA-MB-231
Product
Hoechst 33258 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Place aluminum foil over
the sample to protect it from light and incubate at room temperature for 5-10 minutes.

Optimize incubation time to
minimize signal bleeding through to other channels.

Publication protocol

Measurement of Cell Concentration and DNA Analysis
The final cell concentration within collagen constructs was calculated from standard curves generated using MDA-MB-231 samples of known cell number in increments from 100,000 to 5 million cells. Frozen construct samples were resuspended in 750 μL of 0.1% SDS in PBS and sonicated until homogenous. Samples were diluted using 0.1% SDS in PBS at 1:50 and 1:100 dilutions and 100 μL of each dilution loaded into a microwell plate (Nunc Part No. 237017). Hoescht 33258 stain was added to each well at a concentration of 0.09 μg/mL per well and the plate was analyzed using the Hoescht 33528 protocol on a Modulus Microplate plate reader (Turner Biosystems, Model number 9300–002).

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Discussion

Discussion

2 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - MDA-MB-231 using Hoechst 33258 from Thermo Fisher Scientific.

Paper title
Non-Muscle Myosin II Isoforms Have Different Functions in Matrix Rearrangement by MDA-MB-231 Cells
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Hoechst 33258 below.

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Videos

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