Hoechst 33258

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Place aluminum foil over the sample to protect it from light and incubate at room temperature for 5-10 minutes. Optimize incubation time to minimize signal bleeding through to other channels.

Protocol tips
Place aluminum foil over the sample to protect it from light and incubate at room temperature for 5-10 minutes. Optimize incubation time to minimize signal bleeding through to other channels.

Publication protocol

Measurement of Cell Concentration and DNA Analysis
The final cell concentration within collagen constructs was calculated from standard curves generated using MDA-MB-231 samples of known cell number in increments from 100,000 to 5 million cells. Frozen construct samples were resuspended in 750 μL of 0.1% SDS in PBS and sonicated until homogenous. Samples were diluted using 0.1% SDS in PBS at 1:50 and 1:100 dilutions and 100 μL of each dilution loaded into a microwell plate (Nunc Part No. 237017). Hoescht 33258 stain was added to each well at a concentration of 0.09 μg/mL per well and the plate was analyzed using the Hoescht 33528 protocol on a Modulus Microplate plate reader (Turner Biosystems, Model number 9300–002).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Hoechst 33258 below.

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