Qubit dsDNA HS Assay Kit

Protocol tips

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Do not label the side of the tube as this could interfere with the sample read.

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

DNA quantification and mtDNA purity assessment
All DNA extracts were quantified using the Qubit dsDNA HS assay kit (Life Technologies) according to the suppliers’ protocol. Purity of mtDNA was assessed in duplicate runs of a multiplex qPCR assay targeting a nuclear and a mitochondrial encoded gene to calculate the ratio of mtDNA molecules opposed to nDNA molecules by the relative quantitation method (2ΔCq) as described before44. The percentage of mtDNA in the DNA extract was quantified (eq. 1) based on the ratio mtDNA:nDNA molecules and the sizes of the mitochondrial reference genome (16,569 base pairs, NC_012920) and complete reference genome (haploid 3,088,269,805 base pairs, GRCh38).

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