Cell Cycle Assay Kit (Fluorometric - Green)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
It is not necessary to fix the cells before DNA staining since the Nuclear Green CCS1 is cell- permeable

Upstream tips
It is not necessary to fix the cells before DNA staining since the Nuclear Green CCS1 is cell- permeable

Publication protocol

Cell-cycle analysis
The cells treated by AFB1 after the miRNA inhibitor and NC transfections were harvested and washed once in phosphate-buffered saline, and fixed in 70% ethanol over night. The DNA content was then examined using the Cell Cycle Analysis Kit of Beyotime (Beijing, China) and analyzed using BD FACS Calibur FlowCytometer. The data were analyzed by ModiFit analysis software.



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