Quant-iT™ PicoGreen® dsDNA Assay Kit

Protocol tips

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	To minimize photo bleaching effects, keep the time for fluorescence measurement constant for all samples. During incubation at room temperature, protected from light.

Protocol tips
To minimize photo bleaching effects, keep the time for fluorescence measurement constant for all samples. During incubation at room temperature, protected from light.

Publication protocol

Assessment of hepatocytes viability, proliferation and functionality
AlamarBlue® and PicoGreen® assays
HepG2 metabolic activities, proliferation and growth and scaffold cytotoxicity were assessed using alamarBlue® assay (Molecular Probes®, Invitrogen®) on days 1, 3, 7 and 14. A standard curve was worked out experimentally which suggested 4 h incubation period for the HepG2 cell line to be the optimum timing to measure the FI for alamarBlue®. Further quantification of the cell-line proliferation was made by quantitating double-stranded DNA (dsDNA) using PicoGreen® assay (Quant-iT™ PicoGreen® dsDNA Assay Kit, Molecular Probes®, Invitrogen®) on culture D10 of experiments on PMS and on culture D7 and D14 of experiments on the 3D scaffolds.

FI was measured using a fluorescence plate reader (Fluoroskan Ascent® FL, and Ascent Software, Thermo Labsystems, Helsinki, Finland) at 530 nm excitation wavelength and 620 nm emission wavelength for alamarBlue® and at 485 nm excitation and 538 nm emission for PicoGreen®.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Quant-iT™ PicoGreen® dsDNA Assay Kit below.

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