Qubit dsDNA HS Assay Kit

DNA quantification Human - HeLa

Experiment
DNA quantification Human - HeLa
Product
Qubit dsDNA HS Assay Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

QuBit
Samples were diluted to a 5 ng/μl concentration (estimated by the bioanalyzer results) and quantified using the QuBit dsDNA HS Assay kit and the QuBit 2.0 fluorometer following the manufacturer’s instructions (Life Technologies). Prior to measurement, a five-point calibration curve was established using the supplied standard. One-microliter of the 5 ng/μl solutions were diluted 200-fold in Qubit assay dilution buffer and measured on the fluorometer. Samples that fell below the limit of detection were re-quantified using lower dilutions. Concentrations provided by QuBit were used to calculate the molarity of the initial sample, correcting for the dilution factor and converting to molarity using the average size of the library as detected by the bioanalyzer (Fig. 2). Measures were done in duplicates.

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Discussion

Discussion

2 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - HeLa using Qubit dsDNA HS Assay Kit from Thermo Fisher Scientific.

Paper title
Comparison of DNA Quantification Methods for Next Generation Sequencing
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

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