Qubit dsDNA HS Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not label the side of the tube as this could interfere with the sample read.

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

QuBit
Samples were diluted to a 5 ng/μl concentration (estimated by the bioanalyzer results) and quantified using the QuBit dsDNA HS Assay kit and the QuBit 2.0 fluorometer following the manufacturer’s instructions (Life Technologies). Prior to measurement, a five-point calibration curve was established using the supplied standard. One-microliter of the 5 ng/μl solutions were diluted 200-fold in Qubit assay dilution buffer and measured on the fluorometer. Samples that fell below the limit of detection were re-quantified using lower dilutions. Concentrations provided by QuBit were used to calculate the molarity of the initial sample, correcting for the dilution factor and converting to molarity using the average size of the library as detected by the bioanalyzer (Fig. 2). Measures were done in duplicates.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

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