Qubit dsDNA HS Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not label the side of the tube as this could interfere with the sample read.

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

DNA extraction
DNA extraction from FFPE samples (5–10 slices of 10-μm-thick sections) was performed using NucleoSpin DNA FFPE XS (Macherey-Nagel), all steps were performed according to manufacturer’s instructions. DNA quantitation was carried out with Qubit™ dsDNA HS Assay Kit (Invitrogen, cat. no. Q32854). To obtain useful information, including high-quality sequence data, FFPE DNA samples were treated with NEBNext FFPE DNA Repair Mix (New England BioLabs, cat. no. M6630 L) according to manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

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