QuantiFluor® dsDNA System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Exposure to light will reduce the sensitivity of the assay. Store QuantiFluor® dsDNA Dye and working solution protected from light

Upstream tips
Exposure to light will reduce the sensitivity of the assay. Store QuantiFluor® dsDNA Dye and working solution protected from light

Publication protocol

Gene expression
RNA was extracted with TRI Reagent. In parallel to RNA, the double stranded DNA was quantified using Quantus™ Fluorometer and corresponding QuantiFluor® RNA and dsDNA Systems to exclude possible contamination of RNA with genomic DNA. The concentration of double stranded DNA was always below the detection limit. The isolated RNA was reversed transcribed (High Capacity cDNA Reverse Transcription Kit, Thermofisher Scientific) and cDNA was quantified by real-time PCR (Mic, Bio Molecular Systems) using primers listed in Supplem. Table 1 and Kapa Sybr Fast qPCR Master Mix. ACTB was used as a reference gene.

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Manufacturer protocol

Download the product protocol from Promega for QuantiFluor® dsDNA System below.

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