QIAamp DNA Stool Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA isolation was performed using the QIAamp DNA Stool Kit protocol (Qiagen; Hilden, Germany) according to the manufacturer’s protocol. Briefly, a stool sample was scooped on dry ice, transferred to an Eppendorf tube, and weighted. Approximately 200 mg of stool sample was overlaid with 1 mL InhibitEX Buffer and vortexed thoroughly until homogenization was achieved. The sample was then heated at 95°C for 5 min followed by a centrifugation at 14 000 rpm for 2 min using a MiniSpin Plus centrifuge (Eppendorf; Hamburg, Germany) to pellet stool particles. A sample of the supernatant (200 μL) was transferred into a fresh tube, mixed with 15 μL Proteinase K and 200 μL of AL buffer, and incubated at 70°C for 10 min. Two hundred μL of ethanol was added and DNA was recovered on a QIAamp spin column according to the QIAamp DNA Stool Kit protocol. DNA was eluted then measured with Nanodrop (Thermo-Fisher; Waltham, MA, USA) spectrophotometer and stored in EB buffer at –20°C until further analysis.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Stool Mini Kit below.

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