MethylFlash Methylated DNA 5-mC Quantification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not allow pipette tip to touch the outer edges or inner sides of the wells to prevent solutions from sticking to the surface

Protocol tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to prevent solutions from sticking to the surface

Publication protocol

Quantification of DNA methylation
Total DNA from the cells upon specific treatments was extracted and purified with the DNeasy kit (Qiagen), followed by quality control with a NanoDrop spectrophotometer (Thermo Scientific). The level of global DNA methylation was quantified with the MethylFlash methylated DNA quantification kit (Epigentek). The methylation assay is a 96-well-plate-based colorimetric immunoassay. In each well 100 ng of DNA was attached to the bottom surface and the DNA methylation marks were labeled with monoclonal antibodies. The developed color signals were normalized to that from a standard DNA sample with known methylation percentage. The promoter methylation of selected cancer miRNAs was assessed by a PCR-based EpiTect Methyl II array (EAHS-591Z, Qiagen), following the protocol provided by the manufacturer.

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Manufacturer protocol

Download the product protocol from Epigentek for MethylFlash Methylated DNA 5-mC Quantification Kit below.

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