7-AAD (7-Aminoactinomycin D)
DNA quantification Human - BMDM
Publication protocol
Cell cycle analysisBMDM were infected with for 16 hr as previously described or treated with Bleocin with or without IFN-β, as described above. Cells were then pulsed with BrdU for 30 min using the BrdU-FITC or –APC kits per the manufacturer’s instructions. DNA content was assessed by 7AAD (BD) and data were acquired on a FACSCalibur or BD LSR II (BD Biosciences) and were analyzed with FlowJo software (TreeStar).
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Discussion
Discussion
4 years ago
Author:
Dorota Ostrowska
About nuclease-free water in DNA quantification
Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?
Papers
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Manufacturer protocol
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