Qubit dsDNA HS Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not label the side of the tube as this could interfere with the sample read.

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

High throughput sequencing
Single read library preparation and high throughput single read sequencing for 36 cycles was carried out on an Illumina Genome Analyser IIx according to the manufacturer’s protocols (Illumina) with some modifications. Each immunoprecipitated sample for library preparation was the product of five separate technical replicates of immunoprecipitation pooled together and purified using Qiagen MinElute columns and quantified using the hsDNA Qubit assay (Invitrogen, Life Technologies, California, USA). A 1:20 dilution of Illumina adaptors was used at the adaptor ligation step to avoid adaptor dimer formation and a 1:2 primer dilution used to prevent dimerization during the PCR amplification stage. The samples were quantified using the hsDNA Qubit assay (Invitrogen, Life Technologies, California, USA) and the size range analysed using a HS DNA chip on a 2100 Bioanalyser (Agilent Technologies, West Lothian, UK) prior to submission for qPCR analysis and cluster generation and sequencing by the CSC/IC Genome Core facility.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

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