MethylFlash Methylated DNA 5-mC Quantification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to prevent solutions from sticking to the surface

Upstream tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to prevent solutions from sticking to the surface

Publication protocol

2.11. DNA and RNA methylation
To measure the levels of 5-methyldeoxycytosine (5-mdC) in DNA, 5-methylcytosine (5-mC) in RNA and N6-methyladenosine (m6A) in RNA, ELISA-based assays were used, namely, MethylFlash Methylated DNA 5-mdC Quantification Kit, MethylFlash 5-mC RNA Methylation ELISA Easy Kit and EpiQuik m6A RNA Methylation Quantification Kit, respectively (EpiGentek, Farmingdale, New York, USA).

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Manufacturer protocol

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